Ovis aries Recombinants
- by Jo Miles
Bottom
TGF alpha is a mitogenic polypeptide that can bind to the EGF receptor, EGFR, and act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Methodology/Main Findings
Here we describe the first portion of the mtDNA sequence of a European sheep from the Copper Age. DNA was extracted from the hairs that were part of the clothing of the so-called Tyrolean Iceman or Ötzi (5,350 – 5,100 years before present). Mitochondrial DNA (a total of 2429 base pairs, encompassing a part of the control region, tRNAPhe, a part of the 12S rRNA gene, and the entire cytochrome B gene) was sequenced using a mixed sequencing procedure based on amplification by PCR and sequencing 454 pooled amplification products. We have compared the sequence with the corresponding sequence of 334 extant lineages.
Product name
Recombinant Ovis aries Protransforming growth factor-alpha
Short description: Recombinant protein
Host species: Yeast
Purification: Greater than 90% as determined by SDS-PAGE.
Description of the immunogen: Region of expression: 24-97aa
Sequence information: Extracellular domain
Uniprot: P98135
Accession number: P98135
Calculated MW: 34.9 kDa
Label information: N-terminal with GST label
Formulation: Buffer based on Tris50% glycerol
Storage
Shelf life is related to many factors, storage condition, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of the liquid form is 6 months at -20˚C, -80˚C. The shelf life of the lyophilized form is 12 months at -20˚C, -80˚C.
Notes:
Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
Conclusions/Importance
A phylogenetic network based on a new cladistic notation for the mitochondrial diversity of domestic sheep shows that Ötzi’s sheep belongs to haplogroup B, demonstrating that sheep belong to this haplogroup were already present in the Alps more than 5000 years ago. On the other hand, the Ötzi sheep lineage is defined by two transitions (16147 and 16440) which, taken together, define a motif that has not yet been identified in modern sheep populations.
Bottom TGF alpha is a mitogenic polypeptide that can bind to the EGF receptor, EGFR, and act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Methodology/Main Findings Here we describe the first portion of the mtDNA sequence of a European sheep from the Copper Age. DNA was extracted from the hairs…
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