The multi med Influenza A/B Antigen Test is a rapid chromatographic immunoassay for the qualitative detection of influenza A and B antigens in nasal swab specimens. Its purpose is to aid in the rapid differential diagnosis of influenza A and B viral infections.
Identify samples as acute or convalescent. Acute and convalescent samples must be submitted on separate test request forms.
Note: This test should not be used to detect infection with influenza A H1N1 virus (swine-origin).
Expected response time: 15 days
- Shows: Serum
- Volume: 1ml
- Minimum volume: 0.5ml
- Container: Red Cap Tube or Gel Barrier Tube
- Storage instructions: Refrigerate
Influenza (commonly known as the “flu”) is a highly contagious, acute viral infection of the respiratory tract. It is a communicable disease that is easily spread through coughing and sneezing of aerosolized droplets containing live viruses. Influenza outbreaks occur each year during the fall and winter months. Type A viruses tend to be more common than type B viruses and are associated with the most severe influenza epidemics, while type B infections tend to be milder. The gold standard of laboratory diagnostics is a 14-day cell culture with a variety of cell lines that can support the growth of influenza viruses.
Cell culture is of limited clinical utility, as results are obtained too late in the clinical course for effective patient intervention. Reverse transcriptase-polymerase chain reaction (RT-PCR) is a newer method that is generally more sensitive than culture with improved detection rates over the culture of 2-23%. But nevertheless; RT-PCR is expensive, complex, and must be performed in specialized laboratories. The Influenza A/B Antigen Test qualitatively detects the presence of influenza A and/or influenza B antigens in nasal swab specimens and provides results in 15 minutes. The test uses antibodies specific for influenza A and influenza B to selectively detect influenza A and influenza B antigens in nasal swab specimens.
- Use: Detect antibodies against influenza A and B
- Methodology: Complement fixation (CF)
- Reference interval: Negative: <1:8
Serologic typing is valuable for epidemiology and for planning therapy. Since influenza type A can be treated with amantadine, but influenza type B cannot, this distinction may need to be made.
The Influenza A/B Antigen Test is a qualitative lateral flow immunoassay for the detection of influenza A and influenza B nucleoproteins in nasal swab specimens. In this test, an antibody specific for influenza A and influenza B nucleoproteins is coated separately on the test line regions of the test cassette. During testing, the extracted sample reacts with antibodies against influenza A and/or influenza B that are coated on particles.
The mixture travels up the membrane to react with the influenza A and/or influenza B antibody on the membrane and generate one or two coloured lines in the test regions. The presence of this coloured line in one or both test regions indicates a positive result. To serve as a procedural control, a coloured line will always appear in the control region if the test has been performed correctly.